Abstract
Enicospilus ramidulus (Hymenoptera: Ichneumonidae) is a common parasitoid of Noctuidae larvae, with potential role in natural pest control. Here, we sequenced and annotated the complete mitochondrial genome of E. ramidulus. The mitochondrial genome spans 15,424 bp with an A + T content of 83.7% It comprises 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and a control region (CR). Phylogenetic analyses conducted using the maximum-likelihood method supported a close relationship between E. ramidulus and E. sp. within the subfamily Ophioninae. This study enhances our understanding of the mitochondrial genome structure of E. ramidulus, and contributes to the broader research field of Ichneumonidae genomics.