Abstract
Populus × xiaohei var. gansuensis (C. Wang & H.L. Yang) C. Shang, a dominant tree in western China with high ecological and economic value, has no previously reported chloroplast genome. Here, we first assembled its complete circular chloroplast genome (156,632 bp) via whole-genome sequencing, which contains 133 genes. Phylogenetic analysis places it in the Tacamahaca clade, sister to P. microphylla and P. germanica. This resource aids poplar conservation, genetic research, and enriches Populus genomic data.