Abstract
Hemidactylus bowringii Gray, 1845, a species of the family Gekkonidae, has garnered attention due to its ecological significance and adaptability to diverse environments. The mitochondrial DNA of H. bowringii was packaged as a compact 17,091 bp circular molecule with an A + T content of 53.8%. It contains 37 typical mitochondrial genes, including 13 protein-coding genes, 2 rRNAs, 22 tRNAs, and 1 non-coding region. We reconstructed a phylogenetic tree based on the mitogenome sequences of 17 species and two outgroups (Sphenomorphus indicus and Calotes versicolor). This newly characterized mitogenome provides a foundational resource for conservation genomics and phylogenetic studies of gekkonid lizards.