Abstract
Canine ehrlichiosis, caused by Ehrlichia canis, represents a relevant challenge in veterinary medicine, particularly in resource-limited settings where access to laboratory-based diagnostics may be constrained. This pilot and exploratory study aimed to evaluate the feasibility of a portable electronic olfactometer as a non-invasive screening approach, based on the analysis of volatile organic compounds (VOCs) present in breath, saliva, and hair samples from dogs. Signals were acquired using an array of eight metal-oxide (MOX) gas sensors (MQ and TGS series). After preprocessing, principal component analysis (PCA) was applied for dimensionality reduction, and the resulting features were analyzed using supervised machine-learning classifiers, including AdaBoost, support vector machines (SVM), k-nearest neighbors (k-NN), and Random Forests (RF). A total of 38 dogs (19 PCR-confirmed infected cases and 19 controls) were analyzed, generating 114 samples evenly distributed across the three biological matrices. Among the evaluated models, SVM showed the most consistent performance, particularly for saliva samples, achieving an accuracy, sensitivity, and precision of 94.7% (AUC = 0.964). In contrast, breath and hair samples showed lower discriminative performance. Given the limited sample size and the exploratory nature of the study, these results should be interpreted as preliminary; nevertheless, they suggest that electronic olfactometry may represent a complementary, low-cost, non-invasive screening tool for future research on canine ehrlichiosis, rather than a standalone diagnostic method.