Abstract
This study aimed to identify the botanical origins of honey through pollen analysis across agro-ecologies of Kelala district, South Wollo, Ethiopia. Fifteen honey samples were collected from traditional beehives, with 5 samples from each of the highland, midland, and lowland agro-ecologies. Qualitative and quantitative pollen analyses revealed that 22 honeybee plants belonging to 8 families, with no families of poisonous origin, and 3 growth forms were identified. Among these plant species, 18 were found in the midland and 13 were recorded in each of the highland and lowland agro-ecologies. The family Fabaceae, with 7 (31.81 %) of the identified honeybee plants, was highly dominant (p < 0.03) compared to the other families. Herbs, with 18 plant species, were highly dominant (p < 0.001) compared to shrubs and trees. In terms of plant species diversity, 10 plant species (Bidens pachyloma, Guizotia scabra, Becium grandiflorum, Eleusine floccifolia, Lens culinaris, Lippia adoensis, Medicago polymorpha, Ocimum basilicum, Trifolium steudneri, and Zea mays) were found in more than 50 % of the honey samples, with the first 2 in all the samples studied. Analysis of each honey sample showed that 8 (53.33 %) of the samples were monofloral, 3 (20 %) were bifloral, and the rest were multifloral. However, all the honeys produced due to agro-ecology (geographical origin) were monofloral. Guizotia scabra in the highland and Bidens pachyloma in the midland and lowland agro-ecologies were the predominant pollen producing species and contributors of monofloral honey. In conclusion, the safe and healthy monofloral honey produced across agro-ecologies suggests the suitability of the honey for human consumption and can potentially attract investors.