Abstract
This study evaluated age-related changes in nutrient digestibility in dogs and examined the effects of physiological development and dietary composition on digestive efficiency. Twenty Beagle dogs were assigned to three groups: puppies (<1 year; n = 8), adults (3-4 years; n = 8), and seniors (10-11 years; n = 4). All animals were fed diets formulated to contain identical nutrient levels that met or exceeded the minimum recommended nutrient requirements established by the Association of American Feed Control Officials. The digestibility of dry matter (DM), crude protein (CP), ether extract (EE), nitrogen-free extract (NFE), calcium (Ca), phosphorus (P), and amino acids were compared among the groups. The results showed that NFE digestibility was significantly higher in puppies, whereas CP digestibility was lower than that in adults and seniors, likely due to immature digestive function. In addition, EE digestibility was significantly lower in puppies, whereas P digestibility decreased with age. No significant difference was observed in Ca digestibility. Amino acid digestibility is lower in puppies, particularly for essential amino acids such as lysine, isoleucine, histidine, and arginine. These results indicate that age-related differences in digestive physiology and protein source affect nutrient utilization, providing a basis for developing life stage-specific nutritional strategies for companion animals.