Single-cell sequencing reveals novel cellular heterogeneity in uterine leiomyomas
单细胞测序揭示子宫平滑肌瘤中新的细胞异质性
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作者:Jyoti Goad, Joshua Rudolph, Mehrdad Zandigohar, Matthew Tae, Yang Dai, Jian-Jun Wei, Serdar E Bulun, Debabrata Chakravarti, Aleksandar Rajkovic
| 期刊: | Human Reproduction | 影响因子: | 6.000 |
| 时间: | 2022 | 起止号: | 2022 Sep 30;37(10):2334-2349. |
| doi: | 10.1093/humrep/deac183 | 研究方向: | 细胞生物学、肿瘤 |
| 细胞类型: | 其它细胞 | |
Methods
We collected five MED12-variant positive leiomyomas and five myometrium samples from a total of eight patients. We then performed single-cell RNA sequencing on freshly isolated single-cell preparations. Histopathological assessment confirmed the identity of the samples. Sanger sequencing was performed to confirm the presence of the MED12 variant in leiomyomas. Main
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