Abstract
BACKGROUND: Hemolytic Disease of the Fetus and Newborn (HDFN) is a significant neonatal condition resulting from ABO and Rhesus (Rh) blood group incompatibility between mother and fetus. Adequate knowledge and positive attitudes among pregnant women toward blood grouping can help prevent complications. This study assessed the Knowledge and Attitude of Pregnant Women on ABO and Rhesus Grouping towards Preventing Neonatal Hemolytic Disease at Rasheed Shekoni Federal University Teaching Hospital, Dutse, Jigawa state. METHODOLOGY: A descriptive cross-sectional study was conducted among 316 pregnant women receiving antenatal care at the hospital. A structured questionnaire was used to collect data on socio-demographic characteristics, knowledge of ABO and Rh blood grouping, and attitude toward their importance in preventing HDFN. Data were analyzed using descriptive and inferential statistics. RESULTS: The majorities of respondents (46.2%) were aged 26-30 years and most had at least a secondary education (57.2%). Awareness of ABO and Rh blood groups was high, with 70.3% of respondents knowing their blood group and Rh status. However, only 16.5% demonstrated good knowledge of HDFN, while 33.2% had poor knowledge. Awareness of preventive measures, including the anti-D immunoglobulin, was low (17.4%). Despite knowledge gaps, attitudes were positive, with 87.7% recognizing the importance of blood grouping in pregnancy, and 98.4% supporting public health campaigns on the topic. Age (p = 0.001), educational level (p = 0.001), occupation (p = 0.001), family income (p = 0.001) and number of pregnancies (p = 0.003) were significantly associated with knowledge levels. Attitude was also significantly associated with age (p = 0.003), educational level (p = 0.003) and family income (p = 0.024). CONCLUSION: While awareness of blood grouping was high, knowledge of HDFN and its preventive measures remained limited. Strengthening antenatal education and implementing targeted awareness programs are essential to improve understanding and ensure the effective prevention of HDFN.