Abstract
Dendrocalamus hamiltonii is a commercially important bamboo species of India, experiencing population depletion due to heavy extraction from natural forests. Nuclear simple sequence repeats (nSSRs) were used to study the genetic diversity and population genetic structure of 19 natural stands of D. hamiltonii distributed across the northeast Himalayas. A total of 68 nSSR primer pairs of D. latiflorus and Bambusa arundinacea have been tested in D. hamiltonii for their transferability, out of which 17 primers showing positive and polymorphic amplification were used for genotyping. A total of 130 alleles were generated in 535 individuals of all the populations using selected primer pairs. The marker analysis indicated that D. hamiltonii populations have maintained a low level of genetic diversity (h = 0.175, I = 0.291) in northeastern region of India. Despite a large proportion of the genetic variation (83.47%) confined within the populations, a moderate level of genetic differentiation (F ST = 0.165) was observed among the populations. The clustering pattern obtained in UPGMA and STRUCTURE analysis revealed that most of the populations were clustered in accordance with their geographical distribution. Two populations (DH03 and DH13) exhibiting significant genetic admixture were identified and recommended for in situ conservation. In addition, six highly diverse populations were also suggested for conservation in different geographical area under study. The study has revealed useful nSSR markers for D. hamiltonii, which were lacking earlier and the information generated herein is of paramount importance in devising programs for species conservation and genetic improvement.