Discovery of proteins associated with a predefined genomic locus via dCas9-APEX-mediated proximity labeling
通过 dCas9-APEX 介导的邻近标记发现与预定义基因组位点相关的蛋白质
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作者：Samuel A Myers, Jason Wright, Ryan Peckner, Brian T Kalish, Feng Zhang, Steven A Carr
| 期刊： | Nature Methods | 影响因子： | 36.100 |
| 时间： | 2018 | 起止号： | 2018 Jun;15(6):437-439. |
| doi： | 10.1038/s41592-018-0007-1 |
Abstract
Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.
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