Abstract
OBJECTIVES: In regenerative dentistry, cell proliferation is crucial for tissue repair and immune response modulation, essential for successful regeneration. The adipose-derived stromal vascular fraction (AD-SVF) shows promise in tissue engineering as an autologous therapy. AD-SVF exosomes, cell-free and resilient in ischemic conditions, offer an ethical and hopeful strategy for dental tissue regeneration and wider regenerative medicine use. Although the enhancement of human dental pulp stem cells (hDPSCs) migratory abilities by AD-SVF exosomes is known, their impact on hDPSC proliferation requires further examination. AIMS: This study explores how AD-SVF exosomes influence hDPSC proliferation. METHODS: AD-SVF exosomes, isolated using size exclusion chromatography and characterized through flow cytometry and nanoparticle tracking analysis, were used to treat hDPSCs at varying concentrations. Proliferation was assessed with the cell counting kit-8 assay. Statistical analysis involved one-way ANOVA, post hoc LSD testing, with significance at 0.05. RESULTS AND CONCLUSION: Isolated AD-SVF exosomes, averaging 103 ± 24 nm, expressed CD9+ and CD63+ markers. The study revealed increased hDPSC proliferation on the 5(th) day post low-exosome treatment, indicating a positive association between AD-SVF exosomes and cell proliferation. These results highlight the potential of AD-SVF exosomes to enhance hDPSC proliferation for dental pulp and broader tissue regeneration.