A monomeric StayGold fluorescent protein
单体 StayGold 荧光蛋白
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作者：Esther Ivorra-Molla #, Dipayan Akhuli #, Martin B L McAndrew, William Scott, Lokesh Kumar, Saravanan Palani, Masanori Mishima, Allister Crow, Mohan K Balasubramanian
| 期刊： | Nature Biotechnology | 影响因子： | 33.100 |
| 时间： | 2024 | 起止号： | 2024 Sep;42(9):1368-1371. |
| doi： | 10.1038/s41587-023-02018-w |
Abstract
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.
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