Abstract
Depending on the macromolecule size and configuration, migration rates through capillary electrophoresis vary greatly. Internal size standards for capillary electrophoresis of the same macromolecule may not be readily available. The Macromolecule Tool in GeneMarker® aids with analysis of macromolecule fragments without an internal lane size standard. Methods included importing raw data files to the software and physically identifying reference peaks in the samples known to have the same size. The program uses this information to calibrate from one capillary to another. Characteristics of the aligned data (such as relative size, peak height, peak area, peak ratios) were exported in an excel sheet. Ninety six raw data files from 4 dye capillary electrophoresis were analyzed. Peak height, height ratio, area, area ratio, and relative sizes were determined for all samples. These values can be used to determine characteristics such as number and relative size of degradation products or other macromolecules, such as DNA binding carbohydrates commonly functioning in gene regulation.