Abstract
Preterm pre-labor rupture of membranes (PPROM) increases maternal and neonatal sepsis risk, yet its association with maternal microbial translocation and systemic inflammation remains unclear. We investigated whether PPROM is linked to microbial DNA in maternal blood (MB) and inflammatory responses around delivery. In 66 PPROM patients (median GA: 32±1 weeks), MB was collected pre-delivery and within 1 hour postpartum. Fetal membranes (FM) and placental tissues were sampled immediately after delivery. Bacterial load and diversity were analyzed via 16S rDNA qPCR and sequencing. Maternal cytokines were quantified by multiplex immunoassay, and fetal inflammatory exposure (Triple I) assessed using histological chorioamnionitis (HCA), cord blood haptoglobin, and IL-6. Bacterial DNA was detected in maternal blood (MB) pre- and post-delivery, with a significant postpartum decline (p=0.004). FM carried higher bacterial load and biodiversity than placenta (p<0.001), dominated by Mycoplasma spp. Maternal IL-6 and IL-10 levels rose postpartum (p<0.05), particularly in cases with fetal inflammatory exposure. Limited overlap was found between MB and tissue microbial taxa. In conclusion, bacterial DNA is detectable in maternal circulation in patients with PPROM before birth but rapidly clears postpartum alongside a robust cytokine surge, suggesting efficient clearance and dynamic inflammatory changes.