Superparamagnetic cobalt ferrite nanoparticles as T(2) contrast agent in MRI: in vitro study

超顺磁性钴铁氧体纳米粒子作为MRI中的T2对比剂:体外研究

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Abstract

Superparamagnetic cobalt ferrite nanoparticles (CoFe(2)O(4)) possess favourite advantages for theranostic applications. Most of previous studies reported that CoFe(2)O(4) magnetic nanoparticles (MNPs) are suitable candidates for induction of hyperthermia and transfection agents for drug delivery. The present study synthesized and investigated the potential use of CoFe(2)O(4) as a contrast agent in magnetic resonance imaging (MRI) by using a conventional MRI system. The CoFe(2)O(4) were synthesized using co-precipitation method and characterized by TEM, XRD, FTIR, EDX and VSM techniques. Relaxivities r(1) and r(2) of CoFe(2)O(4) were then calculated using a 1.5 Tesla clinical magnetic field. The cytotoxicity of CoFe(2)O(4) was evaluated by the MTT assay. Finally, the optimal concentrations of MNPs for MRI uses were calculated through the analysis of T(2) weighted imaging cell phantoms. The superparamagnetic CoFe2O4 NPs with an average stable size of 10.45 nm were synthesized. Relaxivity r(1),(2) calculations resulted in suitable r(2) and r(2)/ r(1) with values of 58.6 and 51 that confirmed the size dependency on relaxivity values. The optimal concentration of MNPs for MR image acquisition was calculated as 0.154 mM. Conclusion: CoFe(2)O(4) synthesized in this study could be considered as a suitable T(2) weighted contrast agent because of its high r(2)/r(1) value.

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