Abstract
Wound damage must be shielded from inflammatory responses caused by the external environment, while also receiving adequate moisture and promoting wound healing. Cinnamomum verum, Coleus aromaticus/amboinicus, and Mentha have proven effects on anti-inflammatory and antimicrobial effects as separate in various studies, but no studies have observed the synergistic effects of one herb on the other. The aim of the present study is to assess the anti-inflammatory and antimicrobial properties of karpooravalli, mint, and cinnamon formulation against wound pathogens for better healing of mouth ulcers through an in vitro study for further mouth paint preparation. Herbal extract is prepared using herbal plants karpooravalli, mint, and cinnamon. We studied the antimicrobial effects on Escherichia coli, Staphylococcus aureus, and Pseudomonas with ampicillin antibiotic disks as positive control and anti-inflammatory with control of diclofenac sodium. The antimicrobial activity of zone of inhibition increased with higher concentration of 100 μL against E. coli, S. aureus, and Pseudomonas at 20 mm, 18 mm, and 15 mm, respectively. The anti-inflammatory activity showed the highest percentage of inhibition using 50 μL with 91.5%; this reveals good anti-inflammatory activity by the synergistic action of the herbal formulation. The synergistic effect of the herbal formulation had a comparable anti-inflammatory activity as that of the standard. Hence, it can be employed in large-scale production and may be used for fabricating a natural product based on mouthwashes and mouth paints.