Salivary Cortisol and Total Antioxidant Capacity (TAC) as Biomarkers of Stress in Dental Medicine Students-A Pilot Study

唾液皮质醇和总抗氧化能力 (TAC) 作为牙科医学生压力的生物标志物 - 一项初步研究

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作者:Otilia Bolos, Vanessa Bolchis, Ramona Dumitrescu, Vlad Tiberiu Alexa, Berivan Laura Rebeca Buzatu, Anca Marcu, Catalin Marian, Paula Diana Ciordas, Daniela Jumanca, Atena Galuscan, Octavia Balean

Conclusions

The State-Trait Anxiety Inventory (STAI) scores confirmed elevated anxiety levels during exams. The findings suggest that academic stress negatively impacts TAC while triggering a moderate increase in salivary cortisol, underlining the need for stress management interventions in dental education.

Methods

This study included 44 participants from the Faculty of Dental Medicine at Victor Babes University, Romania. Saliva samples were collected during a low-stress period and prior to exams (high-stress period).

Results

Cortisol and TAC levels were measured using ELISA and TAC assays, respectively, decreasing from an average of 3.69 (SD ± 1.49) before stress to 2.86 (SD ± 2.28) during high-stress periods (p < 0.05), while salivary cortisol levels showed a non-significant change from 23.69 (SD ± 35.6) ng/mL to 20.44 (SD ± 23.07) ng/mL; additionally, female participants exhibited a mean STAI score of 91.65 (SD ± 5.13) compared to 91.09 (SD ± 9.41) for males, indicating greater stress responses among females. Conclusions: The State-Trait Anxiety Inventory (STAI) scores confirmed elevated anxiety levels during exams. The findings suggest that academic stress negatively impacts TAC while triggering a moderate increase in salivary cortisol, underlining the need for stress management interventions in dental education.

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