High-resolution optical genome mapping unveils gene disruptions in a reciprocal chromosomal translocation and severe male infertility: a case report

高分辨率光学基因组图谱揭示相互染色体易位和严重男性不育症中的基因紊乱:病例报告

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Abstract

PURPOSE: This case report describes a 36-year-old male with primary infertility, associated with a balanced reciprocal translocation between chromosomes 1 and 7. The translocation was initially identified through constitutional karyotyping and further characterized using optical genome mapping (OGM). METHODS: Optical genome mapping (OGM) an advanced genomic tool with high-resolution structural variant detection capabilities was used to further characterize the translocation. RESULTS: OGM pinpointed specific breakpoints within the ASB17 gene on chromosome 1 and the INHBA-AS1 gene on chromosome 7, with potential regulatory effects on MSH4 and GLI3, genes involved in meiotic recombination and reproductive development. CONCLUSIONS: This report explores the potential roles of these genes in spermatogenesis, discusses chromatin decondensation and interchromosomal effects as additional infertility mechanisms, and underscores the clinical value of OGM in reproductive genetics.

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