Abstract
1. The effects of external Cs(+) on magnocellular neurosecretory cells were studied during intracellular recordings from 93 supraoptic nucleus neurones in superfused explants of rat hypothalamus. 2. Bath application of 3-5 mM Cs(+) provoked reversible membrane depolarisation and increased firing rate in all of the neurones tested. Voltage-current analysis revealed an increase in membrane resistance between -120 and -55 mV. The increase in resistance was greater below -85 mV than at more positive potentials. 3. Voltage-clamp analysis showed that external Cs(+) blocked the hyperpolarisation-activated inward current, I(H). Under current clamp, application of ZD 7288, a selective blocker of I(H), caused an increase in membrane resistance at voltages < or = -65 mV. Voltage-current analysis further revealed that blockade of I(H) caused hyperpolarisation when the initial voltage was < -60 mV but had no effect at more positive values. 4. Current- and voltage-clamp analysis of the effects of Cs(+) in the presence of ZD 7288, or ZD 7288 and tetraethyl ammonium (TEA), revealed an increase in membrane resistance throughout the range of voltages tested (-120 to -45 mV). The current blocked by Cs(+) in the absence of I(H) was essentially voltage independent and reversed at -100 mV. The reversal potential shifted by +22.7 mV when external [K(+)] was increased from 3 to 9 mM. We conclude that, in addition to blocking I(H), external Cs(+) blocks a leakage K(+) current that contributes significantly to the resting potential of rat magnocellular neurosecretory cells.