Assessment of Salivary Interleukin-6 and Cortisol Levels in Patients with Oral Lichen Planus: A Biochemical Correlation with Clinical Severity

评估口腔扁平苔藓患者唾液中白细胞介素-6和皮质醇水平:与临床严重程度的生化相关性

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Abstract

BACKGROUND: Oral lichen planus (OLP) is a chronic inflammatory mucocutaneous disorder with autoimmune and psychosomatic implications. Recent studies suggest that pro-inflammatory cytokines and stress-related hormones may play a pivotal role in disease pathogenesis. METHODS: A total of 60 participants were recruited, including 40 clinically and histopathologically confirmed OLP patients and 20 healthy controls. OLP patients were further categorized into mild (n = 15), moderate (n = 15), and severe (n = 10) based on the REU (reticulation/erythema/ulceration) scoring system. Unstimulated saliva samples were collected and analyzed using ELISA for IL-6 and cortisol. Statistical analysis was conducted using ANOVA and Pearson correlation. RESULTS: The mean salivary IL-6 levels were significantly higher in OLP patients (78.32 ± 18.56 pg/mL) compared to controls (32.45 ± 10.11 pg/mL, P < 0.001). Similarly, cortisol levels were elevated in the OLP group (6.21 ± 1.84 ng/mL) versus controls (3.14 ± 1.09 ng/mL, P < 0.001). A positive correlation was observed between REU scores and both IL-6 (r = 0.71, P < 0.001) and cortisol levels (r = 0.66, P < 0.001), indicating an association between biomarker levels and disease severity. CONCLUSION: Elevated salivary IL-6 and cortisol levels in OLP patients correlate positively with clinical severity, supporting their potential role as noninvasive biomarkers for monitoring disease activity and psychological stress involvement in OLP pathogenesis.

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