Mutational landscapes of NITD008-resistant EV71 variants revealed through population sequencing

• The mutation spectra between WT population and NITD008-resistant populations during replication are compared. • The 2C protein may be a new target of NITD008 as a nucleotide analogue against EV71 infection. • Antiviral effect of NITD008 against EV71 is associated with increased frequency of U-to-A and U-to-C mutations.