Abstract
Long non-coding RNAs (lncRNAs) have been recognized as key modulators in stomach adenocarcinoma (STAD). This study investigated the functional and clinical relevance of LINC01614 in STAD. 120 patients with STAD were enrolled. The levels of LINC01614 and miR-129-2-3p were quantified by qRT-PCR. Prognostic relevance was evaluated using Kaplan-Meier curve and Cox regression. The regulatory relationship of molecular was assessed via Pearson correlation, bioinformatic prediction, and dual-luciferase reporter assays. Proliferation and motility were evaluated through CCK-8 and Transwell assays, with co-transfection experiments performed to assess their functional interplay. LINC01614 was upregulated, whereas miR-129-2-3p was reduced in both STAD tissues and cell lines. Elevated LINC01614 levels were strongly related to advanced pathological TNM, lymph node metastasis, and reduced survival prognostic, identifying it as an independent prognostic marker. Dual-luciferase assays confirmed that LINC01614 directly binds to miR-129-2-3p, and their expression levels exhibited a significant inverse correlation. Functionally, silencing LINC01614 inhibited cell proliferation, migration, and invasion, while co-transfection with a miR-inhibitor partially reversed these cellular activities. LINC01614 is a potential marker of prognosis in STAD and propels tumorigenesis through its interaction with miR-129-2-3p. LINC01614 may represent a novel molecular target for STAD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10616-025-00870-z.