Abstract
INTRODUCTION: Advanced glycation end products (AGEs) are non-enzymatically formed through reactions between sugars and proteins. Over the past decades, accumulating evidence has linked AGEs to the acceleration of aging and various pathological processes. Despite their biological significance, AGEs remain largely unrecognized by the general public. Therefore, new initiatives are needed to raise awareness of AGEs. METHODS: An AGE colorimetric sheet was developed. The glycation process of collagen with saccharides was analyzed using the sheet under various conditions, along with the physical properties of the protein. RESULTS: The glycation process of collagen depended on time, dose, and temperature. Additionally, we identified a correlation between AGE levels and the mechanical toughness of the collagen matrix. Furthermore, commercially available beverages containing fructose significantly accelerated collagen glycation. CONCLUSION: Our data demonstrate that the novel colorimetric sheet is a practical tool for assessing AGE formation without requiring specialized analytical instruments. This experimental system can enhance public health awareness, contributing to advances in preventive medicine and promoting healthy aging.