Abstract
Objectives: This study aimed to characterize the basic structure of sialylated glycopeptide (SCP) from edible bird's nest, and to explore the intervention effect and mechanism of SCP based on a mouse lung inflammation model induced by lipopolysaccharide (LPS). Methods: C57BL/6 mice were randomly divided into the control group (CON), model group (LPS), EBN group, SCP group, and SA group. Results: The results showed that SCP had the typical structures of polypeptides and carbohydrates. SCP effectively intervened in the lung inflammation response. The number of neutrophils (Neu) in BALF decreased by 41.3%, the level of tumor necrosis factor-α (TNF-α) decreased by 36.4%, and the W/D ratio of lung tissues decreased by 27.2%, effectively preventing pathological changes in lung tissues. A total of 40 differential metabolites such as choline, linolenic acid, and xanthine were screened between the SCP group and the LPS group. These differential metabolites were mainly enriched in the metabolic pathways of glycerophospholipids, alpha-linolenic acid, and purines. Conclusions: The research results support that SCP, as the active substance of edible bird's nest, can effectively improve lung inflammation, providing theoretical guidance for the development of functional edible bird's nest products.