Abstract
The analysis of tissue cultures requires a sophisticated integration and coordination of multiple technologies for monitoring and measuring. We have developed an automated research platform enabling independent devices to achieve collaborative objectives for feedback-driven cell culture studies. Our approach enables continuous, communicative, non-invasive interactions within an Internet of Things (IoT) architecture among various sensing and actuation devices, achieving precisely timed control of in vitro biological experiments. The framework integrates microfluidics, electrophysiology, and imaging devices to maintain cerebral cortex organoids while measuring their neuronal activity. The organoids are cultured in custom, 3D-printed chambers affixed to commercial microelectrode arrays. Periodic feeding is achieved using programmable microfluidic pumps. We developed a computer vision fluid volume estimator used as feedback to rectify deviations in microfluidic perfusion during media feeding/aspiration cycles. We validated the system with a set of 7-day studies of mouse cerebral cortex organoids, comparing manual and automated protocols. It was shown that the automated protocols maintained robust neural activity throughout the experiment while enabling hourly electrophysiology recordings during the experiments. The median firing rates of neural units increased for each sample, and dynamic patterns of organoid firing rates were revealed by high-frequency recordings. Surprisingly, feeding did not affect the firing rate. Furthermore, media exchange during a recording did not show acute effects on firing rate, enabling the use of this automated platform for reagent screening studies.