Abstract
AIM: To evaluate and compare the sealing ability of Mineral Trioxide Aggregate, Biodentine, and bioceramic based root repair material Bio-C Repair when used as a furcation perforation repair material. MATERIALS AND METHODS: In the present study 30 human lower first molars were used. Collected teeth had no caries or restoration, and none had fused roots. Every molar had an endodontic access cavity made utilizing a high-speed long-shank round bur for the initial entry and an Endo-Z for lateral extension and finishing the cavity walls. Each canal orifice was covered with a temporary filling material. The pulpal floor and cavity walls of every molar were thoroughly quoted with two successive coats of clear nail polish. A significant perforation was made between the orifices to the formation area using high speed, long shank round bur #4. The perforation centered between the medial and distal orifice. They were divided into three experimental groups:- Group I: 10 molars repaired using MTA, Group II: 10 molars repaired using Biodentine, Group III: 10 molars repaired using Bio C Repair. Moist cotton pellets were placed over the repair materials, and molars were kept in 100% humidity for 24 h to allow materials to set and later nail varnish was applied to all. Then, according to each group, molars were put in Petri dishes. Methylene blue dye was applied inside the access cavity of all samples for 24 h. Molars were placed under running tap water for 30 min to remove all residues of methylene blue, and then varnish was removed with a Parker blade #15 and polishing discs. Molars were placed in vials containing 1 mL of concentrated (65 wt%) nitric acid for 3 days. Vials were centrifuged at 14,000 rpm for 5 min. The supernatant from each sample was transferred in a quantity of 200 L to a 96-well plate. Sample absorbance was read by an automatic microplate spectrophotometer at 550 nm using concentrated nitric acid as a blank. Statistical analysis was performed using one-way analysis of variance. When the analysis of variance test was significant, the pairwise comparison of the means was done using a post hoc test. The significance level was set at P < 0.05. Statistical analysis was performed with SPSS 14.0 (SPSS Inc., Chicago, IL, USA) for Windows (Microsoft, Redmond, WA, USA). RESULTS: Mean microleakage seen in Group I: 1.56, Group II: 1.23, Group III: 1.48. Intergroup comparison between Group I, Group II, Group III showed statistically significant difference (P < 0.001). CONCLUSION: Within the limitations of the study, it can be concluded that Biodentine showed maximum sealing ability followed by BIO-C and then MTA.