Abstract
European foulbrood (EFB) is a bee larvae-specific infectious disease and the causative pathogen is Melissococcus plutonius. Broad-spectrum antibiotics have classically been used in many countries to control the pathogens; however, their use in apiaries was discontinued in several countries due to concerns regarding the health of bees and humans. Therefore, the development of alternative treatments for use in apiaries that are safe for bees and humans is essential. The present study examined the effects of nisin A supplementation using artificially reared honey bee larvae infected by M. plutonius strains. The results obtained showed that a non-purified nisin A product was toxic to honey bee larvae, while semi-purified nisin A by removing low-molecular-weight (< 3,000) chemicals was not lethal to honey bee larvae. A larval diet supplemented with the semi-purified nisin A significantly increased the survival rate of larvae infected by M. plutonius. The levels of nisin A required for this rescue differed between the M. plutonius strains used for infection, and 12.5 and 100 µg/mL were required for strain DAT606 belonging to clonal complex 3 and strain DAT561 belonging to clonal complex 12, respectively. This beneficial effect was attributed to the antagonistic activity of nisin A against M. plutonius strains, and the levels of viable M. plutonius strains significantly decreased in the larval gut at the required concentrations. Due to the risks associated with the use of antibiotics in apiaries, food-grade nisin A is a promising alternative to control EFB.