Abstract
BACKGROUND: Bone marrow-derived cell (BMDC) transplantation is increasingly recognized as a valuable strategy in regenerative therapies. However, the data regarding the impact of BMDC transplantation on the severity of diabetes mellitus type 1 (DMT1). AIM: This study aimed to determine the effectiveness of adoptive transfer of BMDC isolated from either non-diabetic mice (nBMDC) or diabetic mice (dBMDC) in the treatment of a DMT1 mouse model. METHODS: Male Swiss albino mice underwent a 16-hour fasting period, followed by administration of streptozotocin (STZ) at a dose of 40 mg/kg body weight for five days to induce DMT1. After 14 days, diabetic mice were divided into four groups. The first group served as a diabetic control and received sodium citrate buffer, while the remaining three groups were treated for two weeks with one of the following: subcutaneous (s.c.) administration of insulin (8 U/kg/day), intravenous (i.v.) inoculation of nBMDCs (1 × 10(6) cells/mouse/once), or i.v. injections of dBMDC (1 × 10(6) cells/mouse/once). RESULTS: STZ-induced DMT1 mice treated with either nBMDC or dBMDC exhibited a noticeable increase in the expression of CD4(+) T lymphocytes, CD8(+) T lymphocytes, and NK lymphocytes, myeloid granulocytic neutrophil (CD11b(+)/Ly-6G(+) cells), monocytic macrophage (CD11b(+)/Ly-6G(−) cells), and monocytic CD11b(+)/Ly-6G(−) cells. Additionally, there was a marked reduction in the percentages of CD25(+) T cells, Foxp3(+) cells, regulatory T cells (Tregs), CD25(+)/Foxp(3)(+) cells, Treg CD25(+)/Foxp3(−) cells, Treg CD4(+)/CD25(+) cells, and Treg CD4(+)/CD25(−) cells within the myeloid cell population in the spleen. The therapeutic efficacy of BMDC as an anti-diabetic therapy was further supported by a significant reduction in the proportion of early and late apoptosis in splenic leukocytes, accompanied by a slight increase in necrosis in STZ-induced DMT1 mice receiving either nBMDC or dBMDC. CONCLUSION: In summary, adoptive transfer of BMDCs exerts significant immunomodulatory and anti-apoptotic effects on pancreatic β cells, which are beneficial for pancreatic islets in DMT1 mice. Clinical studies are warranted to evaluate the safety and effectiveness of the adoptive transfer of BMDCs as a potential therapy for autoimmune DMT1.