Abstract
Chlamydomonas reinhardtii is an ideal model organism for studying the cytoplasmic preassembly of ciliary dyneins. However, under normal liquid-culture conditions, Chlamydomonas preassembly-deficient mutants often show no cilia or a small number of ciliated cells (i.e., low ciliation ratio), which hinders further analysis of both ciliary dyneins and the phenotypes of these mutants. In this brief report, we present a modified culture method for one of the Chlamydomonas preassembly-deficient mutants, pf23 . This method enables researchers to obtain enough pf23 cilia for small-scale biochemical, biophysical, structural and phenotypic analyses.