Abstract
Effective hemostasis in deep and irregular wounds remains a critical clinical challenge. To address this, we developed a bioresorbable chitosan composite hydrogel reinforced with short eggshell membrane (ESM) nanofibers, which were obtained through cryogenic grinding. The resulting ESM/CCS hydrogel, crosslinked with citric acid, exhibited significantly enhanced properties compared to pure CCS hydrogel, including a 63% increase in mechanical strength, a two-fold improvement in shape memory, a 25.31% reduction in hemolysis, over 2% higher cytocompatibility, and more than 48% greater hemostatic efficiency. Structural characterization confirmed the successful integration of bioactive chitosan with collagen mimetic ESM nanofibers. This biomimetic approach synergistically combines mechanical reinforcement with biological functionality, highlighting its strong potential as an advanced hemostatic dressing for complex wound management.