Abstract
In this study, we report the draft genome sequence of P. aeruginosa strain PA-Mosul-25, belonging to sequence type 357 (ST357), isolated from a hospital environmental surface in Nineveh, Iraq. The strain was isolated from a swab taken from the floor using a sterile cotton swab. The isolate was cultured on Pseudomonas Agar Base, which is a selective medium, followed by identification using the VITEK 2 system. The whole-genome sequencing was conducted using the Illumina NovaSeq X platform. The assembled genome has a total size of 6157,064 bp, organized into 124 contigs, with an N50 value of 126,162 bp and a GC content of 66.71 %. Genome annotation predicted 5673 protein-coding sequences, 53 tRNA genes, and 3 rRNA operons. Multilocus sequence typing classified the isolate as ST357. Genome analysis identified four β-lactam resistance genes including bla (VEB-9), bla (OXA-10), bla (OXA-50), and bla (PAO), and nine additional antimicrobial resistance genes including aac(6')-Il, aadA1, sul1, dfrB2, fosA, tet(A), catB7, OqxB, and qacE. Comparative analysis showed high genomic similarity to P. aeruginosa WH-SGI-V-07,060 based on ANI and dDDH values. The genome sequence data of P. aeruginosa PA-Mosul-25 have been deposited in the National Center for Biotechnology Information under accession number JBUYZG000000000.1. These data provide a resource for further comparative genomic and antimicrobial resistance studies of ST357 isolates.