Comparison of two DNA extraction methods for the detection of zoonotic protozoan pathogens in oysters and seawater

比较两种DNA提取方法在牡蛎和海水中检测人畜共患原生动物病原体方面的应用

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Abstract

We compared freeze-thaw and bead beating methods for extracting DNA from parasites in oysters and seawater. Bead beating performed better for extracting Cryptosporidium, Giardia, and Toxoplasma in seawater, while freeze-thaw yielded comparable or better detection for Cryptosporidium and Giardia in oysters. This comparison highlights relative strengths and limitations of these methods.

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