Abstract
The use of medicinal plants as an effective source of therapeutic compounds has become increasingly urgent due to the spread of serious diseases, particularly those caused by resistant pathogenic bacteria. In this study, the bioactivity of ginseng oil extract exposed to ultraviolet (UV-C) radiation for 0, 30, and 60 min was evaluated. Ginseng oil extract exhibited potent antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, and Candida albicans, but not Aspergillus niger at 0, 30, and 60 min. The MICs of the ginseng oil extract after UV-C exposure at 0, 30, and 60 min were 125, 62.5, and 31.25 µg/ml for B. subtilis; 125, 125, and 62.5 µg/ml for S. aureus; 250, 125, and 31.25 µg/ml for K. pneumoniae; 125, 62.5, and 15.62 µg/ml for S. typhi; 62.5, 15.62 and 15.62 µg/ml for C. albicans, respectively. Ginseng oil extract exposed to UV-C for 60 min showed the lowest MBC values compared to exposure at 0 and 30 min. Different concentrations of ginseng oil extract showed anti-inflammatory activity compared to sodium dodecyl sulfate as a standard, in addition to anti-biofilm activity compared to blank and control at different time intervals of UV-C exposure. Based on GC-MS analysis, ginseng oil extract contains 24 components for 0 and 30 min, while it contains 29 components for 60 min. The dominant components are hexadecanoic acid methyl ester; 9,12-octadecadienoyl chloride; n-hexadecanoic acid; 9,12-octadecadienoic acid (Z, Z) methyl ester; 9-octadecenoic acid ethyl ester; 9,12-octadecadienoic acid; 9-octadecenoic acid; oleic acid; 9,12-octadecadienoic acid (Z, Z)-; 2-hydroxy-1-(hydroxymethyl) ethyl ester; and (+)-Sesamin. Different concentrations of ginseng oil extract exhibited anti-inflammatory activity compared with sodium dodecyl sulfate (standard), as well as anti-biofilm activity relative to blank and untreated controls across the various UV-C exposure times.