Abstract
Brunfelsia brasiliensis (B. brasiliensis) (Spreng.) L.B.Sm. & Downs is a perennial evergreen shrub that is widely cultivated as an ornamental plant in tropical and subtropical regions. This study presents the complete chloroplast genome of B. brasiliensis, having a length of 169,062 bp. The chloroplast genome contains 137 genes, including 92 protein-coding, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis reveals that Petunia hybrida and P. exserta are closely linked to B. brasiliensis in evolutionary terms. This first chloroplast genome assembly is a valuable resource for future genetic and molecular biology studies of the genus Brunfelsia.