Abstract
Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer for which there is a critical need to identify novel therapeutic targets. Herein we define PSGL-1 as a checkpoint inhibitor using a syngeneic orthotopic model of PDAC. As with PDAC patients, CD8 (+) T cells within murine PDAC tumors expressed high levels of PSGL-1. PSGL-1 (-/-) mice displayed striking T cell-dependent control of primary tumors and lung metastases. Extensive spatial remodeling within PDAC tumors occurred in PSGL-1 (-/-) mice with a dramatic loss of proliferating tumor cells and an increase in CD8 (+) T cell engagement of antigen-presenting cells. The prominent CD8 (+) T cell infiltrates included subsets of pre-exhausted T cells retaining hallmarks of stemness and multifunctional effector capacity. These changes enabled a near complete response of PDAC to therapeutic PD-1 blockade. Our findings identify PSGL-1 as a key regulator of anti-tumor immunity in PDAC, highlighting its potential as a therapeutic target to limit CD8 (+) T cell exhaustion and enhance immunotherapy response. SUMMARY: Hope et al describe a pivotal function of PSGL-1 in CD8 (+) T cell responses to pancreatic ductal adenocarcinoma. Genetic deletion of PSGL-1 elicits tumor control by increasing T cell infiltration and maintaining functional subsets, thereby promoting sensitivity to PD-1 blockade.