Genotyping of the gene polymorphisms FASL 844C/T and FAS 670A/G in patients with idiopathic infertility in Iraq

伊拉克特发性不孕症患者FASL 844C/T和FAS 670A/G基因多态性分型

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Abstract

OBJECTIVE: The failure to conceive following 12 months of unprotected intercourse is referred to as infertility. About 35% of the instances include women and 30% involve men. AIM: The aim of this study was to investigate the gene polymorphisms FASL-844C/T and FAS-670A/G in the promoter region to evaluate susceptibility to male infertility. METHODS: We have investigated single-nucleotide polymorphisms of FAS-670A/G gene and FASL-844C/T gene in 100 subjects. A total of 50 male patients with infertility constituted the infertility group and another group consisting of 50 apparently healthy individuals comprised the control group. RESULTS: The prevalence rate of FAS-670A/G gene polymorphism was statistically significant among both azoospermia and oligospermia subgroups, the homozygous mutant genotype GG (p=0.014 and p=0.043), and mutant allele G (p=0.001 and p=0.004), respectively. Also, FASL-844C/T gene polymorphism was statistically significant among both azoospermia and oligospermia subgroups, the homozygous mutant genotype TT (p=0.032 and p=0.032), and mutant allele T (p=0.007 and p=0.002), respectively. CONCLUSIONS: FAS homozygous mutant genotype (GG) and FASL homozygous mutant genotype (TT) act as etiologic factors in male infertility.

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