Complete genome sequence and genomic characterization of Microcystis panniformis FACHB 1757 by third-generation sequencing

通过第三代测序对 Microcystis panniformis FACHB 1757 进行完整基因组序列分析和基因组表征

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作者:Jun-Yi Zhang #, Rui Guan #, Hu-Jun Zhang, Hua Li, Peng Xiao, Gong-Liang Yu, Lei Du, De-Min Cao, Bing-Chuan Zhu, Ren-Hui Li, Zu-Hong Lu

Abstract

The cyanobacterial genus Microcystis is well known as the main group that forms harmful blooms in water. A strain of Microcystis, M. panniformis FACHB1757, was isolated from Meiliang Bay of Lake Taihu in August 2011. The whole genome was sequenced using PacBio RS II sequencer with 48-fold coverage. The complete genome sequence with no gaps contained a 5,686,839 bp chromosome and a 38,683 bp plasmid, which coded for 6,519 and 49 proteins, respectively. Comparison with strains of M. aeruginosa and some other water bloom-forming cyanobacterial species revealed large-scale structure rearrangement and length variation at the genome level along with 36 genomic islands annotated genome-wide, which demonstrates high plasticity of the M. panniformis FACHB1757 genome and reveals that Microcystis has a flexible genome evolution.

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