Abstract
BACKGROUND: Amide proton transfer (APT), a specific type of chemical exchange saturation transfer (CEST) MRI, has proved valuable in tumor diagnosis and characterization by detecting mobile protein/peptides in cancerous tissues. However, T(1) confounds CEST measurements, leading to reduced specificity to amides and potential misinterpretation of APT imaging. PURPOSE: The study aimed to investigate the feasibility of the quasi-steady-state (QUASS)-based apparent exchange-dependent relaxation (AREX) analysis in correcting T(1) for unbiased tumor APT MRI at 3T. MATERIALS AND METHODS: CEST MRI experiments were conducted on an egg white phantom and on prospectively enrolled brain tumor patients with T(1) values modulated by gadolinium (Gd). QUASS algorithm was employed to reconstruct steady-state Z spectra. Conventional T(1)-uncorrected CEST effect was quantified with a multipool Lorentzian function from QUASS Z spectra. The non-QUASS AREX and QUASS-based AREX with T(1) correction were calculated from the inverse of non-QUASS and QUASS Z spectra, respectively. The student's t-test and Bland-Altman plots were performed to assess the statistical difference and consistency between pre- and post-Gd measurements. RESULTS: In the phantom study, vials with different T(1) values showed conspicuous discrepancy on the conventional uncorrected APT and non-QUASS AREX maps, but comparable contrast on the QUASS-based AREX map. In the human study, 13 patients were enrolled. The contralateral normal-appearing white matter exhibited no substantial change in T(1) and similar CEST effect between uncorrected APT, non-QUASS AREX, and QUASS-based AREX pre- and post-Gd (all P > .05). However, the tumor regions showed significantly reduced T(1) post-Gd that altered the CEST measurements obtained from uncorrected APT and non-QUASS AREX (both P < .001). In comparison, QUASS-based AREX measurements were in excellent agreement between pre- and post-Gd (P = .19). CONCLUSION: QUASS-based AREX analysis can effectively correct T(1) contamination in CEST measurements, facilitating unbiased tumor APT MRI at 3T.