Abstract
Lethe, a predicted lytic bacteriophage with myovirus morphology, was isolated using Mycobacterium smegmatis mc²155 in North Georgia. Lethe has a genome of 155,828 base pairs with 230 predicted ORFs, 34 tRNAs, 1 tmRNA, and 64.70% GC content. Based on gene content, Lethe is assigned to actinobacteriophage cluster C1, sharing up to 99.5% nucleotide identity with members of this cluster.