Abstract
Therapeutic programs involving human-horse interactions are gaining popularity as a means of enhancing human well-being. Understanding the physiological responses of both humans and horses during these interactions is essential for evaluating the effectiveness of such programs. This study examined the effects of specific interactive activities on both humans and horses by monitoring changes in oxytocin and cortisol levels. Six participants and six horses took part in the study. The participants engaged in three distinct activities, each lasting 15 min: (1) resting alone without the horse (resting), (2) standing near the horse without physical contact (standing), and (3) gently rubbing the horse's neck and withers (rubbing). Saliva samples from the participants and blood samples from the horses were collected at three time points for each activity: T0 (before the activity), T1 (at the end of the activity), and T2 (15 min after the activity ended). The results indicated that oxytocin levels significantly increased in horses at T2 following both the standing and rubbing activities, while cortisol levels remained unchanged in both humans and horses across all activities. These findings suggest that human-horse interactions, particularly standing and rubbing, may foster social bonding in horses without eliciting a stress response in either species.