Abstract
Mercury (Hg) is one of the most harmful contaminates posing significant health risks to ecosystems worldwide. Fish, recognized for its affordability and accessibility, serves as a vital source of protein for the global population. To understand the impact of Hg exposure, an experiment was conducted using Barbonymus gonionotus (average weight: 9.64 ± 0.76 g) to determine the median lethal concentration (96 h-LC(50)) and the definitive dose of Hg. This study employed a static, non-renewable bio-assay to assess acute toxicity, using Hg concentrations of 0.3, 0.4, 0.5, 0.6, and 0.7 mg L⁻¹ in the definitive acute toxicity test. These concentrations were further evaluated for their effects on stress and cellular biomarkers, including metallothionein expression, oxidative stress indicators, histopathology, and bioaccumulation. Metallothionein (MT) expression in the liver was evaluated at 48 and 96 h, while oxidative stress markers were assessed in the liver, gill, kidney, and brain tissues. Additionally, glycolytic enzyme activity in the liver, gill, muscle, and kidney, as well as protein metabolic enzymes in the liver, gill, and kidney, were examined over the 96-hour exposure period to understand the effects of Hg at varying concentrations on B. gonionotus. Histopathological changes in the liver and gill and observed, along with the bioaccumulation of Hg in experimental water and different fish tissues. The study concluded that acute Hg exposure caused significant adverse effects on metallothionein expression, stress biomarkers, and the cellular and metabolic activities of B. gonionotus.