Abstract
Nonsense-mediated mRNA decay (NMD) has an important role in the pathoetiology of cancer, including colorectal cancer (CRC). Identification of the lncRNAs associated with this function would enhance our understating about the molecular mechanisms of CRC and facilitate design of novel therapies. In the current study, we used a bioinformatics approach to find NMD-associated lncRNAs in CRC. Then, we assessed expression of these genes in 43 paired CRC samples and adjacent non-tumor (ANT) tissues. Three transcripts were significantly overexpressed in tumor tissue: PABPC1L (mean fold-change ≈ 5.20, 95 % CI 2.30-11.85, P = 0.0003), SNHG17 (mean fold-change ≈ 5.46, 95 % CI 2.04-14.50, P = 0.001), and SNHG1 (mean fold-change ≈ 5.82, P = 0.0086). RUSC1-AS1 showed a non-significant trend toward upregulation (fold-change ≈ 2.15, 95 % CI 0.92-5.00, P = 0.07). The combined four-gene model demonstrated moderate discriminatory power, yielding an AUC of 0.76 (95 % CI 0.65-0.86, p < 0.0001) with balanced sensitivity and specificity of 69.8 % each at the 0.5 cutoff (overall accuracy 69.8 %). Taken together, RUSC1-AS1, SNHG17, PABPC1L and SNHG1 can be novel candidates for future diagnostic studies in CRC.