Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant cause of economic loss in the swine industry, yet its control remains challenging in wean-to-harvest sites. This prospective observational study followed 95 wean-to-harvest sites across six U.S. states for one production cycle. Sites were required to be PRRSV-negative or vaccinated with a modified live virus (MLV) and free of major coronaviruses. Outbreaks were defined as RT-qPCR-positive in unvaccinated sites or detection of ORF5 sequences distinct from the MLV strain. Biosecurity data were collected through a survey, and oral fluids were tested every four weeks. PRRS outbreaks occurred in 14/42 nurseries (33.3%), 8/12 wean-to-finish (66.7%), and 35/41 finishers (82.4%), with lineage 1C.5 most frequently detected. In univariate models, higher odds of outbreaks were associated with transporting pigs of unknown status (OR 9.80, 1.73-55.37), rendering (OR 6.47, 1.62-25.84), and employee cohabitation (OR 6.15, 1.51-25.09). Protective factors included exclusive pumping equipment (OR 0.07, 0.01-0.43) and overnight downtime for multi-site workers (OR 0.15, 0.04-0.56). In multivariable models, finisher sites (OR 17.47, 2.44-125.19) and greater swine site density within one mile (OR 1.62, 1.09-2.41) significantly increased outbreak odds. These results support targeted biosecurity practices, helping farmers and the swine industry reduce PRRS outbreaks.