Abstract
The symphylans from Chongqing, Southwest China were investigated and studied for the first time. Four new species of the genus Symphylella, S. obtusa sp. nov., S. yintiaolingensis sp. nov., S. flabella sp. nov., and S. micropora sp. nov., are identified and described. They were compared with similar species in detail. The DNA barcodes for all new species were sequenced and analyzed together with other congeners and the genetic distance analysis further support our morphological determination. In addition, two groups, the isabellae group and oligosetosa group, of the genus Symphylella are proposed based on the pattern of inserted setae on the tergal processes, and their respective species are listed.