Hydrogen and Methane Detection in Breath in Response to Two Different Types of Dietary Fiber and Its Relationship to Postprandial Glucose Concentration in Obese Patients with Type 2 Diabetes and Normoglycemic Subjects

检测呼气中氢气和甲烷含量对两种不同类型膳食纤维的反应及其与肥胖2型糖尿病患者和血糖正常受试者餐后血糖浓度的关系

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Abstract

Background: The aim of this study was to investigate the relationship between postprandial glycemic levels based on flashmonitoring and the production of intestinal hydrogen (H(2)) and methane (CH(4)) gases based on the measurement of the amount of these gases in exhaled air. Materials and Methods: We studied 14 subjects with type 2 diabetes mellitus (T2DM) and 14 individuals without diabetes (control) with two food load tests, including two types of dietary fiber (inulin and guar gum), with the simultaneous determination of gases in exhaled air and the assessment of glucose levels. Results: All subjects in the control group had a significant increase in exhaled H(2). OR for increased hydrogen production in patients with T2DM was 0.17 (95% CI 0.031-0.93, p = 0.043). The level of H(2) in exhaled breath after food load in patients with T2DM was lower than in normoglycemic subjects. There was an inverse correlation between maximum glucose rise and maximum H(2) in exhaled air after food load in normoglycemic subjects (r = -0.569, p = 0.034). Patients with T2DM had direct correlations between the level of CH(4) in exhaled air and the parameters of postprandial glycemia in the lactulose test (p < 0.05). Conclusions: The confirmation of a causal relationship between decreased H(2) production, increased intestinal CH(4) production, and more severe postprandial glycemia may identify new therapeutic targets in the correction of postprandial glycemia in patients with T2DM.

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