Abstract
The accurate and expeditious detection of minute biomolecules within human body fluids holds paramount significance in the advancement of novel electrode materials. In this research, a novel non-enzyme electrochemical sensor was constructed. It was founded on Au@Ni-MOF (Ni(CH(3)CO(2))(2)) hybrids, with Ni(II) (nickel acetate) serving as the precursor. Specifically, [Ni(3)(BTC)(2)](n) (H(3)BTC = 1,3,5-trimesic acid) featuring coordinatively unsaturated Ni(II) sites and decorated with gold nanoparticles was synthesized via an in-situ growth methodology. The Au@Ni-MOF hybrids exhibit outstanding electrochemical and electrocatalytic characteristics, attributable to the meticulous assembly of AuNPs and Ni-MOF. The Au@Ni-MOF (Ni(CH(3)CO(2))(2))/SPCE was fabricated onto the surface of the screen-printed electrode (SPCE). Subsequently, its electrochemical performance was probed for the discrete and concurrent quantification of dopamine (DA) and uric acid (UA) in 0.01 M phosphate-buffered saline through differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Notably, the cathodic peak current manifested a linear correlation with the DA and UA concentrations across an extensive range, spanning from 0.1 µM to 2 mM for DA and from 0.5 µM to 1.5 mM for UA, respectively. This sensor is applicable in non-enzyme sensing of DA and UA. Additionally, the adsorption energy and bond length of the 2D structures of Ni-MOF and Au@Ni-MOF (Ni(CH(3)CO(2))(2)) were ascertained via DFT simulations, thereby affording valuable insights into the interaction mechanisms between biomolecules and the surfaces of these 2D structures.