Abstract
Citrus reticulata is economically important tree fruit crop in Pakistan, fortified with various nutrients and minerals including Vitamin C and secondary metabolites. Nanotechnology is a twenty‐first century science and deals with production of minute particles termed as nanoparticles. In present study, silver nanoparticles (AgNPs) were synthesised through green method by utilising leaves of Olea europea as main reducing and capping agent. The synthesised AgNPs were characterised through UV visible spectroscopy, SEM, and energy dispersive X‐ray. Furthermore, different concentrations of AgNPs (10, 20, 30 ppm) in combination with Thidiazuron (0.5, 1.0 mg/l) were added onto MS medium to study development and secondary metabolites production in callus culture of C. reticulata. Callus induction percentage (96%) was more in 20 ppm AgNPs and 1 mg/l TDZ concentration. Moreover, high total phenolic, flavonoid contents, and antioxidant activity was observed in 20 ppm AgNPs combined with 0.5 and 1 mg/l TDZ. Enzymatic components (SOD, POD and CAT) were increased in MS medium augmented with 30 ppm AgNPs and TDZ. The total protein content (TPC) was significant in callus cultures treated with TDZ only. This study provides the first evidence of green synthesised AgNPs on callus culture developments and further quantification of biochemical profiling in C. reticula.