Abstract
HEV, primarily known for its waterborne transmission, is increasingly recognized for its zoonotic potential, raising public health concerns for individuals in close contact with animals or animal products. This study aims to evaluate the seroprevalence of Hepatitis E Virus (HEV) among slaughterhouse workers in Saudi Arabia and compare it to a control group of blood donors, emphasizing potential occupational risks and associated factors.This comparative cross-sectional study included 239 slaughterhouse workers (study group) and 250 blood donors (control group). HEV IgG antibodies were detected using an in-house ELISA. Sociodemographic data, occupational exposure duration, and animal contact details were analyzed.The HEV seroprevalence was significantly higher in slaughterhouse workers (49.7%) compared to blood donors (22.1%) (p < 0.0001). Age and duration of occupational exposure were strongly predictive of HEV infection, with workers exposed for over one year showing higher odds of seropositivity. Geographic region and type of animal contact showed no significant associations.The findings suggest that prolonged occupational exposure to animals demonstrated increased the risk of HEV infection among slaughterhouse workers. Public health interventions, including improved hygiene measures, health screenings, and potential vaccination, could mitigate the risk of HEV transmission in high-exposure occupations.