Abstract
Porphyra suborbiculata exhibits strong heat tolerance and has considerable commercial potential under rising sea temperatures; however, its bioactive components remain insufficiently explored. In this study, a heat-tolerant new strain of P. suborbiculata (PS-M4), cultivated by the College of Fisheries, was used as the experimental material. Polysaccharides were extracted using an ultrasound-assisted composite enzymatic method, and extraction conditions were optimized through single-factor experiments and response surface methodology, yielding a maximum extraction yield of 12.45 ± 0.09%. Crude polysaccharides were further purified using a purification apparatus, yielding two fractions, designated PSP-I and PSP-II. Preliminary structural characterization showed that PSP-I possessed a weight-average molecular weight (Mw) of 26.149 kDa, a number-average molecular weight (Mn) of 11.267 kDa, and a polydispersity index of 2.321. Monosaccharide composition analysis indicated that PSP-I was predominantly composed of galactose. Fourier transform infrared spectroscopy (FT-IR) revealed typical polysaccharide functional groups, and scanning electron microscopy (SEM) analysis revealed a porous lamellar morphology. In vitro cell-based assays demonstrated that PSP-I significantly alleviated ultraviolet B (UVB)-induced damage in HaCaT cells by reducing intracellular reactive oxygen species (ROS) levels, enhancing antioxidant enzyme activities, inhibiting apoptosis, and downregulating the expression of matrix metalloproteinases (MMPs). These results suggest that PSP-I has potential as a functional ingredient for mitigating UVB-induced skin damage.