Abstract
BACKGROUND: Since root canal sealers are in contact with periradicular tissues, biocompatibility is one of their most important features. There is no available study about the biocompatibility of NSF and CC Sealer that are newly made bioceramic-based sealers. This study aimed to compare the tissue reaction elicited by NeoSealer Flo (NSF), AH26, and ColdCeramic Sealer (CC sealer) in rats. MATERIALS AND METHODS: The sealers were mixed and applied in molds to fabricate sealer discs, which were then implanted in the subcutaneous tissue of the backs of 30 healthy adult Albino Wistar rats. Each rat received three sealer discs and the fourth incision site remained empty as a control group. After 7, 30, and 90 days, the rats were sacrificed. Biopsy samples were evaluated regarding the extent and severity of inflammation, angiogenesis, fibroplasia, and infiltration. Data were analyzed in SPSS software using Kruskal-Wallis and Mann-Whitney U tests. RESULTS: Tissue reaction to NSF was generally severe and increased up to day 30, but slightly decreased at three months, although it was still severe, and significantly greater than the tissue reaction to other sealer types. After one month, all rats in the NSF group showed foreign body reaction and giant cells around sealer particles; while, foreign body reaction was not seen in other groups. Tissue reaction to CC Sealer and AH26 was not significantly different at any point in time (P0.05) and was the highest on day seven and then decreased up to month three. CONCLUSION: According to the present results, the CC Sealer appears to be a biocompatible material; however, NSF showed higher severity and extent of inflammation and triggered higher tissue reaction.